The Spray Effect of Peronema Canescens Jack As a Disinfectant Against the Growth of Staphylococcus aureus on the Surface of Alginate Molds

Abstract:- The jaw impression has the potential for cross-
infection from blood and saliva attached to the irreversible

hydrocolloid (alginate) mold so that infection control is
carried out by washing with running water and
disinfecting. Disinfection of alginate molds with the spray
can use chemical and natural materials. Peronema
canescens jack (P. canescens jack) is found in Indonesia, one
in West Sumatra, and has antibacterial properties. The
sample is tubular with a diameter of 8 mm and a height of
15 mm, consisting of 6 groups, namely the concentration
group of 0.5%, 1%, and 2%, as well as the positive control
group and negative control group. Microbial test by
examining the growth of Staphylococcus aureus (S. aureus)
after being sprayed and stored for 10 minutes and then
analyzed after 24 hours of incubation with a
spectrophotometer. The analysis used the Kruskal-Wallis
test and continued with the Mann-Whitney test. There is
an effect of spraying Peronema canescence jack at a
concentration of 0.5%, which has a more significant
growth inhibition than a concentration of 1% and 2%.
Disinfection using P. canescens jack can inhibit the growth
of S. aureus at a 0.5%

Keywords:- Alginate, Disinfectant, Peronema Canescens
Jack, Staphylococcus Aureus.